Novel Biosensing Platform Using Single Impact Electrochemistry

Introduction

Over the past few years, a "digital" sensing strategy has emerged, utilizing the detection of silver nanoparticles based on impact electrochemistry. This novel approach offers a low limit of detection and is particularly applicable for point-of-care applications. 

Aim & Research Methods

The primary objective of this Master's Thesis project is to explore the nano-impact detection of functionalized silver nanoparticles (AgNPs) on (polysulfide-)modified electrodes of varying sizes. The fabrication of these electrodes will involve state-of-the-art laser patterning, followed by surface modification, e.g., an ultrathin polysulfide layer using cyclic voltammetry. Upon the successful completion of the fabrication process, the initial phase will involve the detection of unmodified AgNPs. Subsequently, the focus will shift to examining functionalized AgNPs using thiol-modified oligonucleotides, aptamers, and antibodies. The ultimate goal is to establish a reliable detection scheme using these modified AgNPs, avoiding the current limitations of the detection method. Detailed information about the final detection mechanism can be discussed in person.

Requirements

1. Background in chemistry, biochemistry, bioengineering or similar 

2. Extensive lab experience 

Possible starting date & further information

For further details and application please contact Sebastian Freko

 

Additional information